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Monday, March 30, 2009

CD4 - Do It Yourself LIT

Tired of flying to Mexico and doing that border crossing? Ever wanted to do your own LIT? Now you can.

$350 includes:


2 centrifuges (you could resell on ebay when you’re done or donate to a science class and take a tax break)

Centrifuge Tubes (for the last spin to get the pellet)

3ml Herparin Tubes for blood collection (Vacutainer – expired but they are OK to use)

Butterfly Syringes for withdrawing blood (Sterile, sealed, 30+)

Gauze pads

Alcohol pads

Tourniquet

Centrifuge tube stand (metal and Styrofoam)

Lactated Ringers (Might be expired now) – this is for washing & diluting the pellet. You can easily get fresh online.

Sterile Saline (enough for a few LITs) in 10ml syringes (ready to use)

Sterile Pipettes

Separation solution/media (fresh, not expired)

Instructions as copied from a medical journal on how to do this procedure. I'd love to give you mine but liability, ya know?

Use at your own risk. I did my own LIT after two trips to Mexico - it's fairly easy to do if you have a good lab background and my process gets the exact same results (or better). If not, keep going to Mexico.

Shipping definitely extra as those centrifuges are HEAVY.

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